- #Build a constrcuct serial cloner how to
- #Build a constrcuct serial cloner install
- #Build a constrcuct serial cloner full
- #Build a constrcuct serial cloner code
- #Build a constrcuct serial cloner windows
A problem with Feature display after a scan when only one Feature was found A problem with the NCBI BLAST server when using non-ASCII characters in Sequence names When pasting a protein sequence in GenBank format (NCBI) a protein sequence window is now correctly created. A problem updating Feature when undoing a modification PCR : if done on "unknown format" sequence, incorrectly generated "Protein" type. Gateway : generated DNA but then when then saved as unknown type Ligation : Sometimes unnecessarily produced degenerate DNA A File type problem associated to Serial Cloner files (breaking compatibility with Sequencher import in particular)
#Build a constrcuct serial cloner windows
A problem with "Select All" in Sequence and Protein windows A bug in the selection of the most used AA when reverse translating A problem with Features indication when the destination vector was antisense (att2-att1 direction) A problem arising with uncommon RE names like R1.BceSIV in the particular enzyme" chooser of the Graphic Map. A bug sometimes preventing proper copy of images (Graphic map, Virtual Cut, shRNA) in the clipboard (Windows specific) A Windows-specific interface bug the the 'Import Codon Bias' window Changed shortcut of 'Copy All Sequences To Multifasta' to instead of Improved parsing of sequences containing 'X' in the nucleotide sequence Parsing of NCBI sequences (Web Access) to take in account changes in NCBI pages. Unique sites are indicated in bold in enzyme chooser windows (Graphic and Restriction Maps) Absent sites are indicated in italic in enzyme chooser windows (Graphic and Restriction Maps) It is now possible to fully resize Sequence windows A menu entry to go to Serial Cloner Youtube Channel (tutorial) A menu entry to go to Serial Cloner Forum Added a shortcut to 'Copy Non Formatted'
#Build a constrcuct serial cloner full
A button to quickly select a full linear insert in the 'Build a Construct' window 'Anchor' and 'Core' domains of Primers are now recognized as Features in Sequence Windows A shortcut (S) to quickly show or mask Features (in the Features tab of Sequence Windows) A toggle button to highlight differences in aligned proteins Display Features in Protein Align (Local Align) Inherit Features when copying/Pasting Protein sequences Non formatted copy of Protein sequence (Copy More… Menu) A rapid switch between a DNA and Protein Align window Inherit Features when reverse-translating a DNA sequence
Scan for peptide Features in Protein Sequences A button/menu to choose particular sites to be displayed in Restriction maps. An option in the Restriction Map window to only translate nucleotides that are in uppercase
#Build a constrcuct serial cloner code
An option in the Preferences to use the Codon code from Codon Usage tables as a Genetic code used for translation An option in the Preferences to select pK value tables to use for pI estimation MacOSX version now distributed as a signed application (Mountain Lion Gatekeeper compatibility) Estimates Isoelectric point of protein sequences (DNA and Protein Sequence Window) Insertion point position when editing a protein sequence Error in definition of PreScission site
#Build a constrcuct serial cloner install
Slow down under certain Windows install due to autosaving Bug in "show info" toggle under Windows and Linux Detection of overlapping Primers in PCR a preference to limit or not the width of Seq & Prot windows (see the available contextual help window to get more details on new functions)
It also allows to generate sense and antisense sequences to be obtained after bi-sulfite conversion. Serial Cloner 2.5 now allows to import codon frequency tables from the internet using a Web interface.
It is also posible to send directly BLAST request at the NCBI and obtain the result inside a Web interface.
#Build a constrcuct serial cloner how to
You will also find a Restriction Enzyme library management interface, Additional tools, like a web browser for direct import of NCBI and EMBL entries, a virtual cutter to prepare restriction analysis or a silent restriction map generator to find how to introduce restriction sites without modifying the translated peptide are also provided. Features are now visible when aligning locally. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. Finally, you can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. PCR-based fragment or synthetic adaptors.
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